rabbit polyclonal anti brain derived neurotrophic factor bdnf  (Proteintech)

Journal: Neural regeneration research

Article Title: Acetyl-11-keto-beta-boswellic acid promotes sciatic nerve repair after injury: molecular mechanism.

doi: 10.4103/1673-5374.339494

Figure Lengend Snippet: Figure 3 ｜ Gene and protein verification in sciatic nerve injury in rats treated with AKBA. (A) mRNA expression levels (relative to GAPDH) of NGFR (A1), NGF (A2), BDNF (A3), and MPO (A4) in the rat sciatic nerve using qPCR. (B) Protein expression levels (relative to GAPDH) of NGFR (B2), NGF (B3), BDNF (B4), and MPO (B5) in the rat sciatic nerve by western blot assay. Data are expressed as the mean ± SD (n = 3 rats/group) and were analyzed using a one-way analysis of variance followed by the least significant difference test. BDNF: Brain derived neurotrophic factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; MPO: myeloperoxidase; NGF: nerve growth factor; NGFR: nerve growth factor receptor; qPCR: real-time polymerase chain reaction.

Article Snippet: Supplier BDNF 1:500 Rabbit DF6387 AB_2838350 Affinity Biosciences (Beijing, China) NGFR 1:500 Rabbit DF6821 AB_2838781 Affinity Biosciences MPO 1:500 Rabbit A1374 AB_2760599 ABclonal Technology (Wuhan, China) NGF 1:500 Rabbit A14216 AB_2761076 ABclonal Technology GAPDH 1:1000 Rabbit Bs-2188R AB_11065664 Bioss Biotechnology (Beijing, China) IL-1β 1:400 Rabbit WLH3903 AB_2894981 Wanleibio (Shenyang, China) Pro-IL-1β 1:500 Rabbit WL02257 AB_2894987 Wanleibio TNF-α 1:500 Rabbit WL01581 AB_2894992 Wanleibio β-Actin 1:1000 Rabbit bs-0061R AB_10855480 Bioss Biotechnology BDNF: Brain derived neurotrophic factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IL-1β: interleukin 1 beta; MPO: myeloperoxidase; NGF: nerve growth factor; NGFR: nerve growth factor receptor; Pro-IL-1β: pro interleukin 1 beta; TNF-α: tumor necrosis factor-alpha.

Techniques: Expressing, Western Blot, Derivative Assay, Real-time Polymerase Chain Reaction

Journal: Neural regeneration research

Article Title: Acetyl-11-keto-beta-boswellic acid promotes sciatic nerve repair after injury: molecular mechanism.

doi: 10.4103/1673-5374.339494

Figure Lengend Snippet: Figure 2 ｜ Genomics analysis after sciatic nerve injury treated with AKBA in rats. (A) Differential gene expression volcano map. (A1) AKBA group vs. Model group. (A2) AKBA group vs. Sham group. (A3) Model group vs. Sham group. (B) KEGG analysis of differential gene expression in the sciatic nerve. (B1) AKBA group vs. Model group. (B2) Heat map of KEGG enrichment analysis of differentially expressed mRNA between the AKBA and Model groups. Each row represents an enriched KEGG pathway, and each column represents differential mRNA expression. The red color indicates that the mRNA was detected in the corresponding KEGG pathway, and vice versa, and the gray color indicates that no enrichment was detected. (B3) The most significantly different gene expression was in the phagosome pathway. A redder color indicates more up-regulation of gene expression in the AKBA group than in the Model group, and a greener color indicates more down-regulation in the AKBA group than in the Model group. (B4) Expression of different genes after normalization (FRKM values). (B5) AKBA group vs. Sham group. (B6) Model group vs. Sham group. Data are expressed as the mean ± SD (n = 5 rats/group). BDNF: Brain derived neurotrophic factor; Cybb: cytochrome B-245 beta chain; C3: complement C3; NGF: nerve growth factor; NGFR: nerve growth factor receptor: MPO: myeloperoxidase; RT1-M2: RT1 class Ib: locus M2; Stx7: syntaxin 7; Tcirg1: T cell immune regulator 1; Thbs2: thrombospondin 2.

Article Snippet: Supplier BDNF 1:500 Rabbit DF6387 AB_2838350 Affinity Biosciences (Beijing, China) NGFR 1:500 Rabbit DF6821 AB_2838781 Affinity Biosciences MPO 1:500 Rabbit A1374 AB_2760599 ABclonal Technology (Wuhan, China) NGF 1:500 Rabbit A14216 AB_2761076 ABclonal Technology GAPDH 1:1000 Rabbit Bs-2188R AB_11065664 Bioss Biotechnology (Beijing, China) IL-1β 1:400 Rabbit WLH3903 AB_2894981 Wanleibio (Shenyang, China) Pro-IL-1β 1:500 Rabbit WL02257 AB_2894987 Wanleibio TNF-α 1:500 Rabbit WL01581 AB_2894992 Wanleibio β-Actin 1:1000 Rabbit bs-0061R AB_10855480 Bioss Biotechnology BDNF: Brain derived neurotrophic factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IL-1β: interleukin 1 beta; MPO: myeloperoxidase; NGF: nerve growth factor; NGFR: nerve growth factor receptor; Pro-IL-1β: pro interleukin 1 beta; TNF-α: tumor necrosis factor-alpha.

Techniques: Gene Expression, Expressing, Derivative Assay

Journal: Nutrients

Article Title: Long-Term Diet Supplementation with Lactobacillus paracasei K71 Prevents Age-Related Cognitive Decline in Senescence-Accelerated Mouse Prone 8

doi: 10.3390/nu10060762

Figure Lengend Snippet: Effects of 43-week Lactobacillus strain administration on the expression levels of brain-derived neurotrophic factor (BDNF) in the hippocampus and cortex of SAMP8. ( A ) Bdnf mRNA levels measured by quantitative PCR ( n = 8 mice per group); ( B ) Western blotting analysis ( n = 4 mice per group) of the protein levels of BDNF; ( C ) Quantification of band intensities in ( B ). Data are expressed as mean ± SEM, * p < 0.05 vs. the control group.

Article Snippet: The membranes were blocked with 3% BSA in Tris-buffered saline with 0.05% Tween-20 for 1 h at room temperature, and incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-brain-derived neurotrophic factor (BDNF; 1:3000; Abcam, Cambridge, MA, USA), rabbit monoclonal anti-cAMP response element binding protein (CREB; 1:2000; Abcam), rabbit monoclonal anti-Ser133-phosphorylated CREB (pCREB; 1:2000; Abcam), and mouse monoclonal anti-β-actin (1:5000; Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, Western Blot

Journal: Nutrients

Article Title: Long-Term Diet Supplementation with Lactobacillus paracasei K71 Prevents Age-Related Cognitive Decline in Senescence-Accelerated Mouse Prone 8

doi: 10.3390/nu10060762

Figure Lengend Snippet: Representative images of BDNF immunofluorescence (green) in the hippocampal dentate gyrus (DG) and CA3 regions of SAMP8. DAPI stained nuclei (blue). Scale bar: 400 μm.

Article Snippet: The membranes were blocked with 3% BSA in Tris-buffered saline with 0.05% Tween-20 for 1 h at room temperature, and incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-brain-derived neurotrophic factor (BDNF; 1:3000; Abcam, Cambridge, MA, USA), rabbit monoclonal anti-cAMP response element binding protein (CREB; 1:2000; Abcam), rabbit monoclonal anti-Ser133-phosphorylated CREB (pCREB; 1:2000; Abcam), and mouse monoclonal anti-β-actin (1:5000; Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Immunofluorescence, Staining